Heterologous surface display on lactic acid bacteria: non-GMO alternative?

Heterologous protein display on the cell surface of lactic acid bacteria mediated by the s-layer protein

BACKGROUND Previous studies have revealed that the C-terminal region of the S-layer protein from Lactobacillus is responsible for the cell wall anchoring, which provide an approach for targeting heterologous proteins to the cell wall of lactic acid bacteria (LAB). In this study, we developed a new surface display system in lactic acid bacteria with the C-terminal region of S-layer protein SlpB ...

Exploring Surface Display Technology for Enhancement of Delivering Viable Lactic Acid Bacteria to Gastrointestinal Tract

Lactic acid bacteria as a surface display platform for Campylobacter jejuni antigens.

BACKGROUND Food poisoning and diarrheal diseases continue to pose serious health care and socioeconomic problems worldwide. Campylobacter spp. is a very widespread cause of gastroenteritis. Over the past decade there has been increasing interest in the use of lactic acid bacteria (LAB) as mucosal delivery vehicles. They represent an attractive opportunity for vaccination in addition to vaccinat...

Lactic acid bacteria: an alternative platform for live vaccine development

Lactic acid bacteria (LAB), a large group of food grade microorganisms, have recently been developed to express reactive antigens, which are intended to be used for vaccination. Immunological effects of these vaccines depend on the amount of the expressed antigenic protein loaded on the strains utilized for the delivery. Several factors including gene choice, cloning vector, and expression syst...

Lactic acid bacteria: a promising alternative for recombinant protein production

Even though the use of Lactic Acid Bacteria (LAB) is well documented for a variety of dairy food fermentation dating back to the earliest written records [1,2], the use of these Gram-positive anaerobic microorganisms as recombinant microbial cell factories has taken place during the last decades. In this context, it is important to note that the enormous potential of these Generally Recognized ...